retained compound - analyte which has been retained on the column, eluted with the volume bigger than the column dead volume.

retention time (adjusted) (t'R) - the retention value obtained by subtracting the dead time from the total retention time.

retention time (tR) - time elapsed between sample introduction and maximum of response.

See retention parameters.

retention volume (adjusted) (V) retention volume corrected by subtracting the dead volume.

retention volume - volume of mobile phase required to elute the peak maximum of a compound; calculated by multiplying the retention time by the flow rate.

reversed-phase chromatography (RPC) - the most common HPLC mode. Uses hydrophobic packings such as octadecyl- or octylsilane ligands bonded to silica or neutral polymeric beads. Mobile phase is usually water and a water-miscible organic solvent such as methanol or acetonitrile. There are many variations of RPC in which various mobile phase additives are used to impart a different selectivity.

See reversed-phase HPLC.