micro LC - refers collectively to techniques in which a column of smaller-than-usual internal diameter (i.d.) is used for separation. In mico-HPLC, columns of 0.5 mm i.d. are used.

minimum plate height - the minimum of the van Deemter curve that results from a plot of H vs u. This value represents the most theoretical plates that can be obtained for a certain column and mobile phase system. Usually occurs at low flow rates.

See total band broadening.

mobile phase - liquid media which continuously flows through the column and carries the analytes. Usually, in LC the mobile phase is a solvent mixture such as methanol and water for reversed phase LC, or hexane for normal phase.

See mobile phases.

modifier - additive that changes the character of the mobile phase. For example, in reversed phase, water is the weak solvent; methanol, the strong solvent, is sometimes called the modifier.