adsorption chromatography - one of the basic LC modes. Relies on the adsorption process to effect the separation. Molecules are retained by their interaction with the surface.

See also basis of the separation process.

adsorption coefficient - (adsorption equilibrium constant, Henry constant) Expressed as a ratio of the analyte excess adsorption to its equilibrium concentration at the very low concentration level. Analyte retention in HPLC is proportional to its adsorption coefficient.

adsorption isotherm - (see also excess adsorption ) in adsorption, a dependence of the analyte amount concentrated due to the surface interactions on the adsorbent surface on the equilibrium concentration of analyte in the bulk solution. The shape of the adsorption isotherm can determine the chromatographic behaviour of the solute: tailing, fronting, overload, etc.

See also adsorption from solutions

affinity chromatrography - a technique in which a biospecific adsorbent is prepared by coupling a specific ligand (such as an enzyme, antigen, or hormone) for the macromolecule of interest to a solid support (or carrier). This immobilised ligand will interact only with molecules that can selectively bind to it. Molecules that will not bind elute unretained. The retained compound can later be released in a purified state.