GENERAL THEORY

Contents

Basis of the separation process
Retention parameters
Band broadening (column efficiency)
Selectivity and Resolution
Band broadening theory (Van Deemter equation)
Multiple path
Analyte diffusion
Kinetics of the mass transfer
Total band broadening
Thermodynamics of HPLC retention
Adsorption from solutions
Basic retention equation
Basic chromatographic relationships
Dead volume
Capacity factor
Capacity factor from partitioning theory
Effect of the temperature Mobile phase effect