Efficient method development and some times successful troubleshooting of certain problems in HPLC mainly depend on the understanding of the retention mechanism and the way how different parameters influence the separation.
In our discussion of the retention dependencies in reversed-phase HPLC we will be using the surface interaction approach. This has been discussed in the "Theory" and "Adsorbents" chapters.
Note, that analyte - eluent interactions in the bulk solution donot have any influence
on the retention