Date

Topic

Text, chapters

Exam

Sept. 4

Intro to Separation Science.  History. Definitions

9

Gas Chromatography basics

chap. 1, 2

11

GC Instrumentation GC Detectors

chap. 2, 7

16

GC theory (thermo and kinetic)

Chap 3

18

GC columns, stat. phases

Chap. 4,5,6,

23

GC-MS, two-dimensional GC,

Chap. 9, 10

25

Head-Space, SPME, other sample introduction techniques

30

SFC (principles, instruments, applications)

Oct. 2

HPLC Introduction, History, definitions

Ch. 1

Midterm

7

Separation Mechanism, HPLC theory

Ch 2

9

Stationary Phases. Geometry of Silica

Ch. 3

14

Chemistry of Silica surface, RP-phases

Ch. 3

16

RP-HPLC. Eluent type, composition effects. Energetic additivity. Structure-retention relationships.

Ch. 4

21

pH effects on analyte retention

23

Eluent composition effects (adsorption)

28

Salt effects, pH-shift, ion-interactions

Midterm

30

NP-HPLC, SEC, IEX

Ch. 5, 6

Nov. 4

LC/MS

Ch. 7, 19

6

HPLC Method Development

Ch 8

11

HPLC Method Development

Ch 8

13

HPLC Method validation

Ch 9

18

HPLC Optimization, AMD (automated method development)

Ch. 10

20

Review of HPLC application in Pharma industry

Ch. 11 - 16

25

Fast LC (Discussion)

Ch 17

 Dec. 2

Chiral separations

Ch 22

4

CZE, CEC (electrodriven separations)

Final