| HPLC METHOD DEVELOPMENT | |||||
| 2-days Hands-on Short Course (Schedule) | |||||
| Optimization of the HPLC Separation of Pharmaceutical Compounds | |||||
Check your proficiency and decide whether you should attend this course or not. |
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| Below are four consecutive runs of an unknown mixture on a typical C18 column (150x4.6 mm) MeCN/Water 20/80 with different mobile phase pH (10 mM Phosphate buffer adjusted with HClO4). | |||||
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Why does the retention of component A increase when the pH is lowered to 4 and then slightly decreases with further lowering the pH? | ||||
| Why do basic components B and C switch their elution order between pH 2.5 and pH 2? | |||||
| Why did the retention of component D decrease its retention when the pH was lowered from 6 to 2.5 ? | |||||
| Why did the basic component E increase its retention when lowering the pH from 2.5 to 2? |
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| Why does the retention of peak F remain unchanged over the whole pH range 2 –6? (Hint: there are 3 explanations) | |||||
| If you want an explanation for these effects, learn how to apply this knowledge for practical method development and learn to do it yourself | |||||
| you should attend this course! | |||||
| In two days participants will have 8 hours of lab work and 8 hours of lectures. You will be working on the development of a practical separation of an unknown mixture and learn how to select the proper starting conditions and how to modify them in the most optimal way to obtain a fast, reliable and rugged separation method. | |||||
| TO REGISTER | WHERE | ||||
| Email your name, company address and
billing details to Dr. Yuri Kazakevich |
Somerset Marriott, Somerset NJ |
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| PRICE | WHEN | ||||
| $1,250 per person: Please register early, maximum
12
participants per session to allow hands-on experiments. Price includes: coffee breaks, lunch, books, and lab supplies. 20% DISCOUNT for GROUP of 3 or MORE |
May 1-2, 2003 |
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